RESUMO
A condition called "cypress mortality" affects forest of Austrocedrus chilensis (D. Don) Pic. Ser et Bizarri in Argentina. Their classic groups of symptoms has been described as a slow process of defoliation that culminating in death of the tree; nevertheless, dying and recently dead trees with abundant foliage are frequently observed in which foliage changes to red. Cinara (Cupressobium) cupressi (Buckton) is considered the agent responsible for reddening this indigenous conifer in Chile. Therefore, the relationship between the presence of C. cupressi and the new aerial symptoms in A. chilensis from Argentina required evaluation. However, Cinara (Cupressobium) tujafilina (del Guercio) also has been reported from this host, and the differentiation of both species of Cinara is time consuming and requires a great expertise because they share many morphologic and microscopic characters. A rapid molecular method of identification of C. cupressi and C. tujafilina is desirable to detect and differentiate them. We report the development and evaluation of a polymerase chain reaction-restriction fragment length polymorphism method based on the mitochrondial cytochrome oxidase I gene to identify C. cupressi and C. tujafilina in colonies of aphids. The first detection of C. cupressi from A. chilensis in Argentina, is reported based on the new method.
Assuntos
Afídeos/genética , Reação em Cadeia da Polimerase/métodos , Animais , Afídeos/classificação , Argentina , Sequência de Bases , Classificação/métodos , Complexo IV da Cadeia de Transporte de Elétrons/química , Complexo IV da Cadeia de Transporte de Elétrons/genética , Haplótipos , Dados de Sequência Molecular , Polimorfismo de Fragmento de Restrição , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
Avaliou-se a transmissão de Passion fruit woodiness virus (PWV) por Aphis gossypii (Glover). Em dois experimentos independentes, o afídeo transmitiu o PWV para maracujazeiros com taxas de 75 por cento e 100 por cento, ao se depositarem oito e doze afídeos virulíferos por planta, respectivamente. No final dos testes, observaram-se, em algumas plantas de maracujá, formas ápteras e ninfas de A. gossypii, sugerindo a colonização dessas plantas pelo afídeo. Esse parece ser o primeiro relato da colonização de Passiflora edulis f. flavicarpa (Deneger) por uma espécie de afídeo.
Assuntos
Animais , Afídeos , Vírus de Plantas , Passiflora/parasitologia , Passiflora/virologiaRESUMO
The transmission of Passion fruit woodiness virus (PWV) by Aphis gossypii (Glover) was evaluated. In two independent experiments, A. gossypii transmitted PWV to passion fruit plants at the rates of 75% and 100%, when eight and twelve viruliferous aphids were deposited by plant, respectively. At the end of the tests, nymphs of A. gossypii were observed in some of the passion fruit plants, suggesting that the aphid species was colonizing the plants. This seems to be the first report of Passiflora edulis f. flavicarpa (Deneger) colonization by a species of aphid.
